LKQCP Irel 1870; MRCS Eng 1869.
Middleton studied as an undergraduate at the University of Durham in 1863. He later undertook medical training at the Royal Manchester School of Medicine at Guy's Hospital. He was prosector of anatomy at Royal Manchester School of Medicine and assistant medical officer to the General Hospital for Sick Children, Manchester. Middleton later went to Somerset where he was admiralty surgeon to Watchet and Littlestoke.