Among Ashmole's papers are:
- Papers of Dr Simon Forman (1552-1611)
- Medical and astrological papers of Dr Richard Napier (1559-1634), Theodoricus Gravius and John Dee (1527-1608)
- Collections - chiefly astrological - of John Booker (1603-1667) and William Lilly (1602-1681)
Ashmole's collection is also strong in heraldry and local history, and to a lesser extent, in Middle English and seventeenth-century poetry. There is also much material by Ashmole himself, including many original documents collected in preparation for his Institutions, Laws and Ceremonies of the Order of the Garter (1672), a work which is still regarded as a fine piece of research.
The full list of reference numbers is as follows: GB 161 MSS. Ashmole 1-8, 18-20, 31, 33-61, 64, 121, 162, 174-6, 178-225, 227-44, 290, 312-13, 321, 328-34, 337, 339-50, 352-60, 362-71, 374, 376-8, 380-440, 487-8, 536-7, 546-9, 576, 580, 698, 745-60, 762-866, 970-2, 1007, 1021, 1089, 1095, 1097-159, 1161-2, 1164, 1216, 1276-91, 1294-6, 1298, 1352, 1374-493, 1496, 1498-501, 1503-28, 1531, 1534, 1536-42, 1567, 1583, 1630, 1662, 1729-32, 1742-4, 1750, 1752, 1758, 1763, 1776, 1782-3, 1787-96, 1800-32, 1834; Ash. Rolls 1-9, 11-12, 14-16, 18-23, 25-8, 30-3, 35-6, 38-47, 49-50, 52-4.